ASO-TURBILATEX

Intended Use:

ASO Turbilatex Test for estimation of antibodies against Streptolysin ‘’O ‘’ by using Latex turbidmertric method.

Summary:

Streptolysin “O” is a toxic immunogenic exoenzyme produced by haemolytic Streptococci of groups A, C and G. Measuring the ASO antibodies are useful for the diagnostic of rheumatoid fever, acute glomerulonephritis and streptococcal infection. Rheumatic fever is an inflammatory disease affecting connective tissue from several parts of human body as skin, heart, joints etc. and acute glomerulonephritis is a renal infection that affects mainly to renal glomerulus.

ASO Turbilatex Test Kit

Principle:

The ASO-Turbilatex is a quantitative turbidimetric test for the detection of anti-steptolysin “O” (ASO) antibodies in human serum/plasma. Latex particles coated with steptolysin “O” are agglutinated when mixed with samples containing ASO. The agglutination causes an absorbance change, dependent upon the ASO contents of the patient sample that can be quantified by comparison from a calibrator of known ASO concentration.

Specification

Methodology Immunoturbidimetry
Reaction Type Kinetic
Reaction Direction Increasing
Sample Type Human Serum or Plasma
R1:R2 Mixing Ratio 4:1
Primary Wavelength 540 nm
Secondary Wavelength NA
Blank Settings Distilled Water
Blank Absorbance Limit <0.300
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 10 µL
Delay Time 10 seconds
Calibrator Available in the Kit Yes
Factor NA
Linearity 800 IU/ml.
Automation Enabled
Pack Sizes 1×50 ml
Kit components Reagent bottles – R1 and R2.
1 Calibrator vial.
Instructions for Use.
Storage Temperature 2- 80C
Shelf life 24 months
Kit dimensions 1×50 ml – 85x105x45 mm
Approx. weight of kit 1×50 ml – 130 gms
Production Capacity 600 mn ml per annum